rabbit polyclonal antibodies against irf6  (Aviva Systems)

Journal:

Article Title: Abnormal skin, limb and craniofacial morphogenesis in mice deficient for interferon regulatory factor 6 ( Irf6 )

doi: 10.1083/ng1903

Figure Lengend Snippet: Genotypic and phenotypic analysis of mice deficient for Irf6. (a) Irf6 gene trap allele (Irf6gt1). The gene trap vector (VICTR48) inserted 36 bp from the splice donor site of intron 1 and contains flanking long terminal repeats (L), a splice acceptor (SA) and donor (SD) sites, the ORF for neomycin (Neo), stop codons (S), polyadenylation site (pA), the phosphoglycerate kinase (PGK) promoter, marker fusion transcript (MFT) and OmniBank sequence tag (OST) fusion transcript (OFT). (b) Genotypic analysis by PCR of genomic DNA derived from E17.5 embryos using primers a and b to detect wild-type (+) and using primers v and b to detect mutant (−) alleles. (c) Gross appearance of wild-type E17.5 embryos. (d) E17.5 embryo that is homozygous for gene trap allele (Irf6gt1/gt1). (e) Protein blot analysis of protein extracts obtained from E17.5 skin from mice with the indicated genotype. Blots were probed with antibodies directed against the indicated protein.

Article Snippet: We generated rabbit polyclonal antibodies against Irf6 (ref. 17 ) and purchased antibodies for Sfn and Ikka (Santa Cruz Biotech).

Techniques: Plasmid Preparation, Marker, Sequencing, Derivative Assay, Mutagenesis

Journal:

Article Title: Abnormal skin, limb and craniofacial morphogenesis in mice deficient for interferon regulatory factor 6 ( Irf6 )

doi: 10.1083/ng1903

Figure Lengend Snippet: Skeletal defects in mice deficient for Irf6. (a) Skeletal structures of E17.5 wild-type (WT) and newborn mutant (M) embryos were stained for bone (alizarin red S) and cartilage (alcian blue). (b) The spine showed equal numbers of vertebrae in wild-type and null embryos. However, vertebrae in the mutant appeared smaller and showed delayed ossification. The mutant tail was substantially shorter. (c) There were equal numbers of ribs in the wild-type and null embryos, but the sternum appeared shorter with delayed ossification. The xiphoid process (xp) was bifid in the null embryo, demonstrating a failure of complete fusion of the thoracic cage. (d) The long bones were slightly shorter in null versus wild-type littermates, but both null and wild-type embryos showed normal surrounding muscle when skin was dissected. Proximal bones of the upper and lower limb were present in the null embryos, but distal structures, notably the digits, were severely abnormal. Forepaws were magnified to display synostosis of digits and absence of distal phalanges. (e) Skulls from wild-type and null embryos in lateral (top), superior (bottom left) and inferior (bottom right) views. Mandibles were removed for inferior views. The mandible in the null embryo was smaller with a narrower angle than in the wild-type, and the snout was also shorter in the null embryo. The palate shelves (p) in the wild-type embryo fuse along the midline, but the null embryo has a cleft palate. The palate shelves of null embryo are posteriorly and laterally displaced, allowing for direct viewing of nasal structures, including the vomer (v). The presphenoid (ps) is absent in the mutant. Similar results were obtained from three skeletal preparations.

Article Snippet: We generated rabbit polyclonal antibodies against Irf6 (ref. 17 ) and purchased antibodies for Sfn and Ikka (Santa Cruz Biotech).

Techniques: Mutagenesis, Staining

Journal:

Article Title: Abnormal skin, limb and craniofacial morphogenesis in mice deficient for interferon regulatory factor 6 ( Irf6 )

doi: 10.1083/ng1903

Figure Lengend Snippet: Histologic and molecular analyses of Irf6-null E17.5 embryos. (a–d) Hematoxylin and eosin–stained sections (a,b) and electron micrographs (c,d) of back skin. Note the presence of keratohyalin granules (KG) in the granular layer and a cornified layer (CL) of wild-type epidermis (c), which are absent in null epidermis (d). Arrows indicate desmosomes. Dye exclusion assays of whole embryos show a functional barrier in wild-type (e) but not in null (f) embryos. Cellular proliferation and programmed cell death were evaluated by BrdU (g,h) and TUNEL (i,j) staining, respectively. Immunofluorescent staining (red, except loricrin is green) for cornified and granular layers (k–m) and for spinous (o–r) and basal (s–v) layers. Proteins against which antibodies were directed are indicated. Nuclei were counterstained with DAPI (blue), and the basement membrane is indicated by the dashed line.

Article Snippet: We generated rabbit polyclonal antibodies against Irf6 (ref. 17 ) and purchased antibodies for Sfn and Ikka (Santa Cruz Biotech).

Techniques: Staining, Functional Assay, TUNEL Assay, Membrane

Journal:

Article Title: Abnormal skin, limb and craniofacial morphogenesis in mice deficient for interferon regulatory factor 6 ( Irf6 )

doi: 10.1083/ng1903

Figure Lengend Snippet: Change in gene expression for markers representative of epidermal differentiation in skin from wild-type versus Irf6 -null E17.5 embryos

Article Snippet: We generated rabbit polyclonal antibodies against Irf6 (ref. 17 ) and purchased antibodies for Sfn and Ikka (Santa Cruz Biotech).

Techniques: Gene Expression, Expressing, Control

Journal:

Article Title: Abnormal skin, limb and craniofacial morphogenesis in mice deficient for interferon regulatory factor 6 ( Irf6 )

doi: 10.1083/ng1903

Figure Lengend Snippet: Epidermal adhesions and desmosomal structure in Irf6-null embryos. (a–f) Hematoxylin and eosin staining for sections of E14.5 frontal head (a,b), E17.5 rostral trunk (c,d) and E17.5 caudal trunk (e,f). Palate shelves elevate and begin to fuse in wild-type mice (a) but not in null embryos (b), where adhesions contribute to crowding of the oral cavity and prevent the palate shelves from elevating. Hindlimbs and tail are joined by epithelial adhesions (compare c with d). Esophagus is closed in null embryo (compare e with f). *: palate shelves; To: tongue; L: hindlimb; T: tail. Adhesion in the heterozygote was observed superficial to the first molar on the left side only (g, arrow). (h,i) Electron micrographs of desmosomes. Immunofluorescent staining (green) for desmocollin (Dsc) (j,k). We did not observe any difference in desmosomal structure and protein between wild-type and null embryos. Nuclei were counterstained with DAPI (blue).

Article Snippet: We generated rabbit polyclonal antibodies against Irf6 (ref. 17 ) and purchased antibodies for Sfn and Ikka (Santa Cruz Biotech).

Techniques: Staining

Journal:

Article Title: Abnormal skin, limb and craniofacial morphogenesis in mice deficient for interferon regulatory factor 6 ( Irf6 )

doi: 10.1083/ng1903

Figure Lengend Snippet: Expression of stratifin and Ikka in skin from Irf6-null E17.5 embryos. (a,b) Protein blot analyses show that stratifin expression (a) is greater in null embryos, whereas Ikka expression (b) does not seem to change. The respective blots were stripped and reprobed with β-actin as a control for loading. (c) Immunostaining shows that stratifin is expressed in the cytoplasm of spinous cells in skin from wild-type E17.5 embryos (c); it remains cytoplasmic but increases along with the expanded spinous layer in the skin from null embryos (d). (e,f) Immunostaining shows that Ikka is expressed predominantly in the basal layer in skin from both the wild-type (e) and Irf6-null (f) embryos. Ikka is localized to the cytoplasm and in the nucleus of some cells. This distribution does not appear to differ in skin from wild-type and Irf6-null embryos.

Article Snippet: We generated rabbit polyclonal antibodies against Irf6 (ref. 17 ) and purchased antibodies for Sfn and Ikka (Santa Cruz Biotech).

Techniques: Expressing, Control, Immunostaining